Please use this identifier to cite or link to this item: http://monografias.ufrn.br/handle/123456789/9484
Title: Isolation, identification and phenotypic characterization of clinical isolates of the Sporothrix schenckii Complex.
Other Titles: Isolation, identification and phenotypic characterization of clinical isolates of the Sporothrix schenckii Complex.
Authors: Costa, Alexandre Soares de Sena
Keywords: Sporothrix spp.;sporotrichosis;cats;zoonosis;subcutaneous mycosis
Issue Date: 5-Nov-2019
Publisher: Universidade Federal do Rio Grande do Norte
Citation: COSTA, Alexandre Soares de Sena. Isolation, identification and phenotypic characterization of clinical isolates of the Sporothrix schenckii Complex. 2019. 33 f. Trabalho de Conclusão de Curso (Graduação em Farmácia) - Departamento de Farmácia, Universidade Federal do Rio Grande do Norte, Natal, 2019.
Portuguese Abstract: Sporotrichosis, caused by fungi belonging to the Sporothrix schenckii complex, is widely distributed around the globe, especially in tropical and subtropical regions. Traumatic inoculation is the usual route of acquisition. This infectious disease used to be restricted to farmers and armadillo’s hunters due to vegetable manipulation injuries. However, studies in Brazil have reported frequent outbreaks associated with feline transmission, characterizing sporotrichosis as a zoonotic infection. This study aimed to isolate, purify and evaluate in vitro virulence factors (colony growth and melanin production at different glucose concentrations), as well as, antifungal susceptibility testing to itraconazole (ITZ) and amphotericin B (AmB) of 40 clinical isolates obtained from patients living in Rio Grande do Norte state, Brazil. In our study, 75% of the patients were female and two clinical forms of infection were predominant, cutaneous-fixed and lymphocutaneous. We also report a case of primary pulmonary sporotrichosis leading to patient’s death. The molecular screening revealed that 12 strains were identified as S. brasiliensis. All the isolates were susceptible to both antifungal drugs tested, with MIC values ranging from 0.0312 to 0.5 μg/mL for ITZ and 0.125 and 1 μg/mL for AmB. There was no remarkable conclusion for colony growth, but a low number of strains produced melanin. Our study shows a prevalence of cases of sporotrichosis in female patients and S. brasiliensis as an etiological agent of infection in the region. In addition, the isolates demonstrated a satisfactory antifungal susceptibility profiling, with low MIC values to the antifungal drugs tested.
Abstract: Sporotrichosis, caused by fungi belonging to the Sporothrix schenckii complex, is widely distributed around the globe, especially in tropical and subtropical regions. Traumatic inoculation is the usual route of acquisition. This infectious disease used to be restricted to farmers and armadillo’s hunters due to vegetable manipulation injuries. However, studies in Brazil have reported frequent outbreaks associated with feline transmission, characterizing sporotrichosis as a zoonotic infection. This study aimed to isolate, purify and evaluate in vitro virulence factors (colony growth and melanin production at different glucose concentrations), as well as, antifungal susceptibility testing to itraconazole (ITZ) and amphotericin B (AmB) of 40 clinical isolates obtained from patients living in Rio Grande do Norte state, Brazil. In our study, 75% of the patients were female and two clinical forms of infection were predominant, cutaneous-fixed and lymphocutaneous. We also report a case of primary pulmonary sporotrichosis leading to patient’s death. The molecular screening revealed that 12 strains were identified as S. brasiliensis. All the isolates were susceptible to both antifungal drugs tested, with MIC values ranging from 0.0312 to 0.5 μg/mL for ITZ and 0.125 and 1 μg/mL for AmB. There was no remarkable conclusion for colony growth, but a low number of strains produced melanin. Our study shows a prevalence of cases of sporotrichosis in female patients and S. brasiliensis as an etiological agent of infection in the region. In addition, the isolates demonstrated a satisfactory antifungal susceptibility profiling, with low MIC values to the antifungal drugs tested.
URI: http://monografias.ufrn.br/handle/123456789/9484
Other Identifiers: 20150120772
Appears in Collections:Farmácia

Files in This Item:
File Description SizeFormat 
ISOLATION_COSTA_2019.pdf662.27 kBAdobe PDFThumbnail
View/Open


This item is licensed under a Creative Commons License Creative Commons