Please use this identifier to cite or link to this item: http://monografias.ufrn.br/handle/123456789/10679
Title: Diluições de amostras: solução para realização de exames laboratoriais em neonatos?
Other Titles: Samples dilution: solution for carrying out laboratory tests in neonates?
Authors: Duavy, Júlia Guedes de Araújo
Keywords: dilution;biochemical tests;serum;plasma;neonates;phlebotomy
Issue Date: 20-Jul-2020
Publisher: Universidade Federal do Rio Grande do Norte
Citation: DUAVY, Júlia Guedes de Araújo. Diluições de amostras: solução para realização de exames laboratoriais em neonatos? 2020. 43 f. Trabalho de Conclusão de Curso (Graduação em Farmácia) - Departamento de Farmácia, Centro de Ciências da Saúde, Universidade Federal do Rio Grande do Norte, Natal, 2020
Portuguese Abstract: Blood collection in children is a challenging reality compared to adults. The low volume of whole blood of a newborn can suffer a strong impact when laboratory tests are required. Laboratory blood spoliation is related to the intensification of physiological anemia of the newborn, which may predispose to the need for blood transfusion. Recognizing the importance of laboratory tests for screening and monitoring of pathologies in neonates, the objective of this study was to investigate serum/plasma dilution as a possible solution to reduce the volume collected in newborns. Blood samples were obtained from 20 adult volunteers. The blood was collected in a tube containing a clot activator and serum gel separator and, also, in a tube containing the ethylenediaminetetraacetic acid (EDTA) anticoagulant. The serum and plasma were diluted in the proportions of 1:2, 1:3, 1:4 and 1:5, and subsequently, analyzing 19 biochemical parameters. For analysis of the effect of dilution on the values in the samples, linear regression of random effects was used (p<0.05). Uric acid, albumin, aspartate aminostransferase, total bilirubin, globulin, total protein and lactate dehydrogenase were stable in both serum and plasma dilutions. These results may indicate that the pre-dilution of serum and plasma samples for these parameters may bring benefits to clinical practice, especially neonatal. However, further studies involving the dilution of serum/plasma and biochemical analyses should be performed to validate this methodology and confirm the results by this study.
URI: http://monografias.ufrn.br/handle/123456789/10679
Other Identifiers: 2016083920
Appears in Collections:Farmácia

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